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Chiral separation of the plant lignan matairesinol by capillary electrophoresis
Author(s) -
Müller Ulrike,
Mrestani Yahya,
Neubert Reinhard,
Dräger Birgit
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700800
Subject(s) - lignan , capillary electrophoresis , chromatography , enantiomer , chemistry , phenylpropanoid , chirality (physics) , organic chemistry , nambu–jona lasinio model , chiral symmetry breaking , physics , quantum mechanics , biosynthesis , enzyme , quark
Lignans are dimeric phenylpropanoid compounds in plants that enjoy increasing medicinal interest because of their phytoestrogen activity. Lignans are chiral compounds and for most natural occurring lignans, chirality is not known. Separation of racemic matairesinol by CE in a non‐coated silica capillary with carboxymethyl‐β‐cyclodextrin as chiral selector in phosphate buffer was successful. Electrolyte and selector concentrations and pH were systematically optimized in order to obtain baseline separation and short analysis times. Matairesinol from safflower fruit was determined as (−)‐enantiomer. Quantitation results for matairesinol with the optimized method after calibration with authentic lignan were very similar to those by HPLC. The limit of detection is 2 μg/mL sample by DAD detection.