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Electrokinetic injection of DNA from gel micropads: Basis for coupling polony technology with CE separation
Author(s) -
Kosobokova Olga,
Gavrilov Dmitri N.,
Khozikov Vyacheslav,
Stepukhovich Andrey,
Tsupryk Andriy,
Pan'kov Sergey,
Somova Olga,
Abanshin Nikolai,
Gudkov Georgiy,
Tcherevishnik Marina,
Gorfinkel Vera
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700790
Subject(s) - electrokinetic phenomena , capillary electrophoresis , electrophoresis , materials science , monolith , microfluidics , chromatography , gel electrophoresis of nucleic acids , capillary action , dna , analytical chemistry (journal) , nanotechnology , chemistry , catalysis , biochemistry , composite material
We propose a novel method for electrokinetic injection of DNA samples into capillaries from nanoliter gel micropads, deposited on glass slides, which are coated with electroconducting film. Theoretical and experimental proof is presented for the proposed method. The method allows efficient and highly precise injection without physical contact between the gel pad and the capillary. Read length of more than 700 bp at Q20 has been reproducibly demonstrated in fused‐silica capillaries using the proposed injection technique. Based on the obtained results we discuss a novel DNA sequencing system which combines DNA amplification and cycle sequencing in arrays of subnanoliter gel micropads and high‐throughput electrophoretic separation in monolith multicapillary arrays.