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Inheritance and alteration of genome methylation in F1 hybrid rice
Author(s) -
Takamiya Tomoko,
Hosobuchi Saeko,
Noguchi Tomotsugu,
Asai Kenji,
Nakamura Eiji,
Habu Yoshiki,
Paterson Andrew H.,
Iijima Hiroshi,
Murakami Yasufumi,
Okuizumi Hisato
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700784
Subject(s) - biology , genetics , genome , inheritance (genetic algorithm) , methylation , oryza sativa , dna methylation , gene , demethylation , transposable element , gene expression
We analyzed the inheritance of DNA methylation in the first filial generation(F1) hybrid of Oryza sativa L. (“Nipponbare”דKasalath”) by restriction landmark genome scanning (RLGS). Most parental RLGS spots were found in the F1, but eight spots (4%) showed abnormal inheritance: seven of the eight spots were missing in the F1, and one was newly detected in the F1. Here we show demethylation at restriction enzyme sites in the F1. We also found a candidate site of stable heterozygous methylation in the genome. These results show the applicability of the RLGS method for analysis of the inheritance and alteration of methylation in F1 hybrid plants.