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System peaks in micellar electrophoresis: I. Utilization of system peaks for determination of critical micelle concentration
Author(s) -
Lokajová Jana,
Hruška Vlastimil,
Tesařová Eva,
Gaš Bohuslav
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700780
Subject(s) - critical micelle concentration , micelle , pulmonary surfactant , chemistry , inflection point , thermodynamics of micellization , analytical chemistry (journal) , aggregation number , thermodynamics , materials science , chromatography , aqueous solution , physics , mathematics , geometry , biochemistry
A new way to determine the critical micelle concentration (CMC) based on the mobilities of system peaks is presented. A general approach for the CMC determination is based on the change of the slope or on finding the inflection point in the plot of a physical property of solution as a function of surfactant concentration. The determination of CMC by system peaks in CE utilizes a “jump” instead of a continuous change in the measured quantity. This phenomenon was predicted by the program PeakMaster, which was modified for simulation of micellar systems. The simulation of the steep change in mobilities of the anionic system peaks showing the CMC value was verified experimentally in a set of measurements, where the concentration of the surfactant was varied while the ionic strength was kept constant. The experimental work fully proved our model. A comparative electric current measurement was carried out. The proposed method seems to offer easier CMC determination as compared to the standard methods.