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Macrocyclic polyamine‐modified poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) monolith for capillary electrochromatography
Author(s) -
Tian Yun,
Yang Fangxing,
Yang Xuemei,
Fu Enqin,
Xu Ying,
Zeng Zhaorui
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700766
Subject(s) - glycidyl methacrylate , monolith , polyamine , capillary electrochromatography , chemistry , polymer chemistry , methacrylate , photografting , polymer , chromatography , organic chemistry , monomer , capillary electrophoresis , catalysis , biochemistry
1,4,10,13,16‐Pentaazatricycloheneicosane‐9,17‐dione (macrocyclic polyamine)‐modified polymer‐based monolithic column for CEC was prepared by ring opening reaction of epoxide groups from poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) (GMA‐ co ‐EDMA) monolith with macrocyclic polyamine. Conditions such as reaction time and concentration of macrocyclic polyamine for the modification reaction were optimized to generate substantial EOF and enough chromatographic interactions. Anodic EOF was observed in the pH range of 2.0–8.0 studied due to the protonation of macrcyclic polyamine at the surface of the monolith. Morphology of the monolithic column was examined by SEM and the incorporation of macrocyclic polyamine to the poly(GMA‐ co ‐EDMA) monolith was characterized by infrared (IR) spectra. Successful separation of inorganic anions, isomeric benzenediols, and benzoic acid derivatives on the monolithic column was achieved for CEC. In addition to hydrophobic interaction, hydrogen bonding and electrostatic interaction played a significant role in the separation process.