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Cationized hydroxyethylcellulose as a novel, adsorbed coating for basic protein separation by capillary electrophoresis
Author(s) -
Yang Runmiao,
Shi Ronghua,
Peng Shuhua,
Zhou Dan,
Liu Hang,
Wang Yanmei
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700715
Subject(s) - coating , capillary action , capillary electrophoresis , adsorption , polymer , electrophoresis , chemistry , chromatography , reproducibility , aqueous solution , protein adsorption , chemical engineering , materials science , organic chemistry , composite material , engineering
We present cationized hydroxyethylcellulose (cat‐HEC) synthesized in our laboratory as a novel physically adsorbed coating for CE. This capillary coating is simple and easy to obtain as it only requires flushing the capillary with polymer aqueous solution. A comparative study with and without polymers was performed. The adsorbed cat‐HEC coating exhibited minimal interactions with basic proteins, providing efficient basic protein separations with excellent reproducibility. Under broad pHs, the amine groups are the main charged groups bringing about a global positive charge on the capillary wall. As a consequence, the cat‐HEC coating produced an anodal EOF performance. A comparative study on the use of hydroxyethylcellulose (HEC) and cat‐HEC as physically adsorbed coatings for CE are also presented. The separation efficiency and analysis reproducibility proved that the cat‐HEC polymer was efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. The long‐term stability of the cat‐HEC coating in consecutive protein separation runs has demonstrated the suitability of the coating for high‐throughput electrophoretic protein separations.