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Affinity monolith preconcentrators for polymer microchip capillary electrophoresis
Author(s) -
Yang Weichun,
Sun Xiuhua,
Pan Tao,
Woolley Adam T.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700704
Subject(s) - monolith , monolithic hplc column , chromatography , capillary electrophoresis , analyte , glycidyl methacrylate , chemistry , electrophoresis , elution , polymer , methacrylate , adsorption , fluorescence , copolymer , high performance liquid chromatography , biochemistry , organic chemistry , physics , quantum mechanics , catalysis
Developments in biology are increasing demands for rapid, inexpensive, and sensitive biomolecular analysis. In this study, polymer microdevices with monolithic columns and electrophoretic channels were used for biological separations. Glycidyl methacrylate‐ co ‐ethylene dimethacrylate monolithic columns were formed within poly(methyl methacrylate) microchannels by in situ photopolymerization. Flow experiments in these columns demonstrated retention and then elution of amino acids under conditions optimized for sample preconcentration. To enhance analyte selectivity, antibodies were immobilized on monoliths, and subsequent lysozyme treatment blocked nonspecific adsorption. The enrichment capability and selectivity of these affinity monoliths were evaluated by purifying fluorescently tagged amino acids from a mixture containing green fluorescent protein (GFP). Twenty‐fold enrichment and 91% recovery were achieved for the labeled amino acids, with a >25 000‐fold reduction in GFP concentration, as indicated by microchip electrophoresis analysis. These devices should provide a simple, inexpensive, and effective platform for trace analysis in complex biological samples.