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Bilayer disk capillary electrophoresis: A novel method to study drug partitioning into membranes
Author(s) -
Boija Elisabet,
Lundquist Anna,
Nilsson Mikael,
Edwards Katarina,
Isaksson Roland,
Johansson Gunnar
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700682
Subject(s) - membrane , partition coefficient , capillary electrophoresis , bilayer , lipid bilayer , chemistry , chromatography , partition (number theory) , capillary action , cationic polymerization , phase (matter) , analytical chemistry (journal) , materials science , biochemistry , mathematics , organic chemistry , combinatorics , composite material
CE in the presence of lipid bilayer disks was introduced as a new approach in membrane partitioning studies. The disks were used as a pseudostationary phase in the partial‐filling mode of CE and the partitioning of cationic drugs was determined. The migration times of the analytes increased linearly with the lipid amount in the system. An appropriate algorithm for the calculation of a partition coefficient is presented. In the disk‐shaped bilayers, which have excellent stability and shelf life, all of the lipids are readily available for interaction and the disks can be used as realistic cell membrane models.