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A sensitive, rapid and specific technique for the detection of collagenase using zymography
Author(s) -
Thanzami Kawl,
Roy Ipsita
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700655
Subject(s) - collagenase , zymography , chromatography , substrate (aquarium) , chemistry , electrophoresis , substrate specificity , enzyme , biochemistry , biology , ecology
A fast and specific method for the detection of collagenase by electrophoresis is described. The method avoids inclusion of the substrate in the resolving gel and can detect nanogram levels of the enzyme.