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Pressure‐assisted capillary electrochromatography with electrospray ionization‐mass spectrometry based on silica‐based monolithic column for rapid analysis of narcotics
Author(s) -
Lu Minghua,
Zhang Lan,
Feng Qiang,
Xia Shifei,
Chi Yuwu,
Tong Ping,
Chen Guonan
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700653
Subject(s) - monolithic hplc column , chromatography , capillary electrochromatography , detection limit , ammonium acetate , chemistry , mass spectrometry , analytical chemistry (journal) , electrochromatography , selectivity , linear range , electrospray ionization , electrospray , volumetric flow rate , capillary action , materials science , high performance liquid chromatography , capillary electrophoresis , biochemistry , physics , quantum mechanics , catalysis , composite material
A pressure‐assisted CEC (pCEC) with ESI‐MS based on silica‐based monolithic column was developed for rapid analysis of narcotics. Combining the extremely high permeability and separation efficiency of silica‐based monolithic column with the high selectivity and sensitivity of pCEC‐ESI‐MS, the developed system exhibited its prominent advantages in separation and detection. A systematic investigation of the pCEC separation and ESI‐MS detection parameters was performed. Experiment results showed that the optimized separation efficiency could be obtained at 8 bar assisted pressure with 25 kV separation voltage, using the solution containing 65% ACN v/v and 20 mmol/L ammonium acetate with pH 6.0 as running buffer. 3 μL/min of sheath liquid was considered as the optimized flow rate since it could provide the maximum signal intensity. Under the optimum conditions, the tested five narcotics could be completely separated within 10 min with the detection limit in the range of 2.0–80 nmol/L. The proposed method has been successfully used for detection of narcotics in real urine samples.