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Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis
Author(s) -
Busnel JeanMarc,
Lion Niels,
Girault Hubert H.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700643
Subject(s) - electrokinetic phenomena , capillary electrophoresis , chromatography , chemistry , resolution (logic) , electrophoresis , micellar electrokinetic chromatography , capillary action , absorbance , analytical chemistry (journal) , microfluidics , materials science , nanotechnology , computer science , artificial intelligence , composite material
Electrokinetic supercharging has been integrated in CZE for the development of a highly sensitive methodology for protein tryptic digest analysis. A careful choice of the experimental conditions led to sensitivity enhancement factors between 1000 and 10 000 whilst maintaining a satisfactory resolution. Peptides in the low nanomolar concentration range have been detected despite the use of the poorly sensitive UV absorbance detection mode. The buffer system used in this study is fully suitable for coupling CE to MS.