Premium
Determination of amino acids by capillary electrophoresis‐electrospray ionization‐mass spectrometry: An evaluation of different protein hydrolysis procedures
Author(s) -
Simionato Ana V. Colnaghi,
Moraes Edgar Perin,
Carrilho Emanuel,
Tavares Marina Franco Maggi,
Kenndler Ernst
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700629
Subject(s) - chemistry , hydrolysis , chromatography , hydrolysate , amino acid , electrospray ionization , capillary electrophoresis , mass spectrometry , electrospray , methanol , capillary electrophoresis–mass spectrometry , organic chemistry , biochemistry
Abstract In this work, a CE equipment, online hyphenated to an IT MS analyzer by a linear sheath liquid interface promoting ESI, was used to develop a method for quantitative determination of amino acids. Under appropriate conditions (BGE composition, 0.8% HCOOH, 20% CH 3 OH; sheath liquid composition, 0.8% HCOOH, 60% methanol; V ESI , +4.50 kV), analytical curves of all amino acids from 3 to 80 mg/L were recorded presenting acceptable linearity ( r >0.99). LODs in the range of 16–172 μmol/L were obtained. BSA, a model protein, was submitted to different hydrolysis procedures (classical acid and basic, and catalyzed by the H + form of a cation exchanger resin) and its amino acid profiles determined. In general, the resin‐mediated hydrolysis yields were overall similar or better than those obtained by classical acid or basic hydrolysis. The resulting experimental‐to‐theoretical BSA concentration ratios served as correction factors for the quantitation of amino acids in Brazil nut resin generated hydrolysates.