Premium
Nonaqueous capillary electrophoresis for analysis of the ethanol consumption biomarker phosphatidylethanol
Author(s) -
Varga Arthur,
Nilsson Staffan
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700548
Subject(s) - phosphatidylethanol , chromatography , chemistry , ethanol , capillary electrophoresis , choline , methanol , phospholipid , extraction (chemistry) , butanol , quantitative analysis (chemistry) , ammonium acetate , sample preparation , membrane , high performance liquid chromatography , biochemistry , phosphatidylcholine , organic chemistry
Nonaqueous CE (NACE) methodology was developed for the separation and determination of phosphatidylethanol (Peth), a new biomarker of ethanol intake. Peth is an abnormal phospholipid formed in cell membranes only in the presence of ethanol, via the transphosphatidylation reaction of phospholipase D. The NACE separation medium consisted of 80 mM ammonium acetate in 50% ACN, 33% 2‐propanol, 12% hexane and 5% methanol. A stacking effect was obtained by reducing the concentration of ammonium acetate in the separation medium for all injected samples. The LOD was estimated to 1 μM (5.6 fmol) of Peth with conventional UV detection, equalling 0.4 μmol/L blood. Peth was successfully determined in extracts of human blood samples. Separation of Peth from other blood lipids in the lipid extract sample was performed in 5 min. The method facilitates smaller sample volumes and performs about ten times faster compared to earlier chromatographical methods.