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Identification of new regional marker proteins to map mouse brain by 2‐D difference gel electrophoresis screening
Author(s) -
Jacobs Sandy,
Van de Plas Babs,
Van der Gucht Estel,
Clerens Stefan,
Cnops Lieselotte,
Van den Bergh Gert,
Arckens Lutgarde
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700487
Subject(s) - difference gel electrophoresis , proteome , microbiology and biotechnology , proteomics , in situ hybridization , biology , two dimensional gel electrophoresis , messenger rna , biochemistry , gene
To screen for new region‐specific protein markers we compared the proteome maps of the primary visual and somatosensory areas V1 and S1 in mouse brain using 2‐D difference gel electrophoresis (2‐D DIGE). Twenty‐three protein spots showed a statistically significant difference in expression level between V1 and S1, with 52% appearing more abundantly in V1. Twenty‐six proteins were mass spectrometrically identified in 22 spots. To assess the validity of this list of potential areal markers generated by 2‐D DIGE, the effective area‐specific distribution profile of creatine kinase brain subtype (CKB), a protein with a clearly higher expression level in S1, was monitored with in situ hybridization. The mRNA expression profile of CKB displayed a clear area‐specific distribution, which allowed demarcation of S1 and its topographical borders with neighboring neocortical areas. This proteomic study demonstrates the innovative application of 2‐D DIGE and MS to select new regional markers for neuroscience research.