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CE‐MS n of complex pectin‐derived oligomers
Author(s) -
Coenen GerdJan,
Kabel Mirjam A.,
Schols Henk A.,
Voragen Alphons G. J.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700465
Subject(s) - chemistry , pectin , oligomer , hydrolysis , monomer , chromatography , capillary electrophoresis , sugar , acid hydrolysis , polymer , molecule , ion exchange , ion , organic chemistry , biochemistry
As pectin molecules are too large and heterogeneous to analyze as a whole, the polymer is usually degraded to smaller oligomers, which are often analyzed by high‐performance anion exchange chromatography (HPAEC). However, the high salt concentration necessary to elute pectin oligomers by HPAEC is incompatible with online mass detection. To overcome such a disadvantage, a CE‐IT‐MS system was set up to further elucidate the fine structure of charged oligosaccharides. An effective separation of differently substituted galacturonic acid containing oligomers was obtained by low‐pH CE‐LIF analysis. By adapting the buffer and capillary online MS detection was enabled. Moreover, with MS/MS it was possible to localize sugar residues' substitutions. With this combined CE‐MS approach LIF electropherograms of xylogalacturonan and rhamnogalacturonan I digests could be annotated. The method was further exemplified by a complex oligomer mixture of acid hydrolyzed apple pectin, which was separated and characterized by CE‐MS n . Oligomers present in low amounts could be localized by their corresponding m/z , as was demonstrated by selected mass range representation.