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Speciation of magnesium in rat plasma using capillary electrophoresis‐inductively coupled plasma‐atomic emission spectrometry
Author(s) -
Deng Biyang,
Li Xianfeng,
Zhu Pingchuan,
Xu Xiangshu,
Xu Qiumei,
Kang Yanhui
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700423
Subject(s) - magnesium , chemistry , capillary electrophoresis , inductively coupled plasma , inductively coupled plasma mass spectrometry , chromatography , analytical chemistry (journal) , electrolyte , mass spectrometry , plasma , physics , organic chemistry , electrode , quantum mechanics
A new method for speciation analysis of magnesium species and quantification of free magnesium concentrations in rat plasma was developed by on‐line coupling of CE with inductively coupled plasma‐atomic emission spectrometry (ICP‐AES). Baseline separation of seven magnesium species was achieved by using a 120 cm (100 μm internal diameter) fused‐silica capillary, a 20 kV separation voltage and a solution of 50 mmol/L NaAc‐HAc (pH 5.5) as electrolyte buffer. CE‐ICP‐AES analysis of a rat plasma sample showed the presence of seven magnesium species, one of which was identified as free Mg 2+ ion by spiking a Mg 2+ standard; the migration time of the Mg 2+ peak in the standard and the spiked sample matched with each other. One protein‐bound magnesium species in rat plasma is associated with albumin, and the other three species are combined with globulin. The concentration of free magnesium in the plasma was 14.0 mg/L. The other six magnesium species were estimated to be 4–15 mg/L. RSDs of migration time and peak area for the magnesium species from ten replicates were less than 5%. The developed method was also applied to speciation analysis of magnesium species in spiked plasma samples. The recoveries of the free magnesium species in four samples ranged from 95.8 to 103.8%.