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Determination of trace levels of benzodiazepine in urine using capillary electrochromatography‐time of flight mass spectrometry
Author(s) -
Blas Maximilien,
McCord Bruce R.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700422
Subject(s) - chromatography , chemistry , mass spectrometry , detection limit , capillary electrochromatography , capillary electrophoresis
This paper details a method for the separation and determination of ten benzodiazepines in urine using CEC‐MS(TOF) and an hexyl acrylate‐based porous monolith. The TOF mass spectrometer provides an exact mass of protonated benzodiazepines to three decimal places (1–6 ppm). This high selectivity along with the CEC separation, provides an effective method for the identification of benzodiazepines. Linearity is satisfactory for all compounds in the concentration range of 25–500 ng/mL for lorazepam and 12.5–500 ng/mL for the others. The RSDs are between 1.4–2.3% for retention times and 1.1–9.2% for relative areas. Using the monolithic stationary phase, a preconcentration step is achievable and permits an 75–140‐fold improvement in sensitivity. This strategy permits the quantification of these drugs down to 1 ng/mL in urine. This method was used for the analysis of benzodiazepines in spiked urine samples.