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Hippocampal signaling cascades are modulated in voluntary and treadmill exercise rats
Author(s) -
Chen WeiQiang,
Viidik Andrus,
Skalicky Monika,
Höger Harald,
Lubec Gert
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700336
Subject(s) - hippocampal formation , medicine , phosphoprotein , signal transducing adaptor protein , transcription factor , endocrinology , signal transduction , biology , microbiology and biotechnology , chemistry , biochemistry , phosphorylation , gene
Systematic protein expression studies in the brain of exercising and sedentary animals have not been carried out for far. Signaling proteins are main structures regulating hippocampal function and we decided to determine differences in signaling protein levels in rat hippocampus by a proteomic approach. Aged, male Sprague–Dawley rats, 23 months old, were used for the study: the first group consisted of sedentary rats, the second of rats with voluntary exercise from 5 to 23 months and the third was performing involuntary exercise on a treadmill from 5 to 23 months. 2‐DE with subsequent mass spectrometrical identification of spots followed by quantification of spots was carried out. Annexin A5, A3, phosphatidylethanolamine‐binding protein, guanine nucleotide‐binding protein G(I)/G(S)/G(T), 14‐3‐3 protein gamma, 14‐3‐3 protein zeta/delta, prohibitin, visinin‐like 1, protein phosphatase 1, septin 8, phosphoprotein enriched in astrocytes 15, transcription factor Pur‐beta, EEA1 protein, SH3 domain‐binding glutamic acid‐rich‐like protein 2, and cell division cycle 42 showed differential protein levels in the three groups. These results form the basis for functional studies elucidating mechanisms and links between exercise and hippocampal signaling and function.