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Further improvement of hydrostatic pressure sample injection for microchip electrophoresis
Author(s) -
Luo Yong,
Zhang Qingquan,
Qin Jianhua,
Lin Bingcheng
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700330
Subject(s) - chromatography , sample (material) , hydrostatic pressure , electrophoresis , hydrostatic equilibrium , chemistry , materials science , mechanics , physics , quantum mechanics
Hydrostatic pressure sample injection method is able to minimize the number of electrodes needed for a microchip electrophoresis process; however, it neither can be applied for electrophoretic DNA sizing, nor can be implemented on the widely used single‐cross microchip. This paper presents an injector design that makes the hydrostatic pressure sample injection method suitable for DNA sizing. By introducing an assistant channel into the normal double‐cross injector, a rugged DNA sample plug suitable for sizing can be successfully formed within the cross area during the sample loading. This paper also demonstrates that the hydrostatic pressure sample injection can be performed in the single‐cross microchip by controlling the radial position of the detection point in the separation channel. Rhodamine 123 and its derivative as model sample were successfully separated.