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Evaluation of band broadening in chemiluminescence detection coupled to pressurized capillary electrochromatography with an off‐column coaxial flow interface
Author(s) -
Lin Zian,
Lin Jian,
Wu Xiaoping,
Lin Xucong,
Xie Zenghong
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700327
Subject(s) - chemistry , chemiluminescence , luminol , chromatography , reagent , analytical chemistry (journal) , capillary electrochromatography , detection limit , analyte , capillary action , capillary electrophoresis , organic chemistry , materials science , composite material
A system of off‐column coaxial flow chemiluminescence (CL) detection coupled to pressurized CEC (pCEC) was described. The interface utilized a reactor that introduced postcolumn CL reagent into the capillary effluents in a sheathing flow profile. To compare and evaluate band broadening of analytes caused by the detector, the typical CL compounds luminol and N ‐(4‐aminobutyl)‐ N ‐ethylisoluminol (ABEI) were separated and detected by pCEC or capillary HPLC (cHPLC) coupled to CL and UV detector, respectively. The results demonstrated that the band broadening caused by off‐column detection interface was minimized due to the fast kinetic nature of the CL reaction. With the proposed pCEC‐CL system, the detection limits of luminol and ABEI were 1.0×10 −8 and 8.0×10 −8 mol/L, respectively, which were approximately 100‐fold more sensitive than those obtained with UV absorption. In addition, separation and detection of the ABEI‐labeled L ‐lysine ( L ‐Lys) and L ‐arginine ( L ‐Arg) were accomplished by pCEC‐CL method based on the principle of ABEI‐potassium ferricyanide‐alkaline medium CL reaction system. Under the optimum conditions, good results could be achieved compared with pCEC‐UV.