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Simultaneous enantioseparation and sensitivity enhancement of basic drugs using large‐volume sample stacking
Author(s) -
Deñola Nerissa L.,
Quiming Noel S.,
Saito Yoshihiro,
Jinno Kiyokatsu
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700299
Subject(s) - electrokinetic phenomena , analyte , chromatography , stacking , chemistry , sensitivity (control systems) , analytical chemistry (journal) , materials science , organic chemistry , electronic engineering , engineering
Simultaneous enantioseparation with sensitive detection of four basic drugs, namely methoxamine, metaproterenol, terbutaline and carvedilol, using a 20‐μm ID capillary with native β‐CD as the chiral selector was demonstrated by the large‐volume sample stacking method. The procedure included conventional sample loading either hydrodynamically or electrokinetically at longer injection times without polarity switching and EOF manipulation. In comparison to conventional injections, depending on the analyte, about several hundred‐ and a thousand‐fold sensitivity enhancement was achieved with the hydrodynamic and the electrokinetic injections, respectively. The simple method developed was applied to the analysis of racemic analytes in serum samples and better recovery was achieved using hydrodynamic injection than electrokinetic injection.