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Ultra‐fast simultaneous analysis of genetically modified organisms in maize by microchip electrophoresis with LIF detector
Author(s) -
Kumar Kailasa Suresh,
Kang Seong Ho
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700273
Subject(s) - ethidium bromide , genetically modified maize , chromatography , ethylene oxide , chemistry , electrophoresis , fluorescence , resolution (logic) , genetically modified organism , matrix (chemical analysis) , analytical chemistry (journal) , genetically modified crops , transgene , polymer , biochemistry , dna , physics , organic chemistry , quantum mechanics , artificial intelligence , computer science , copolymer , gene
This study examined the potential of microchip electrophoresis (ME) with a LIF detector using a programmed field strength gradient (PFSG) in a conventional glass double‐T microchip for the ultra‐fast detection and simultaneous analysis of genetically modified (GM) maize. The separation efficiency and sensitivity at various sieving gels (poly(ethylene oxide) (PEO, M r 8 000 000) and 2‐hydroxyethylcellulose (HEC) ( M r 250 000)) and fluorescent dye concentrations were investigated. The PCR products of both the GM and non‐GM maize were analyzed within 30 s under the PFSG (470.6 V/cm for 20 s, 117.6 V/cm for 12 s, and 470.6 V/cm for 30 s) with a 2.5% HEC sieving matrix in the running buffer, 1×Tris‐borate EDTA (TBE) (pH 8.30) and 0.5 ppm ethidium bromide. The five transgenic maize varieties (Event176, MON810, Bt11, GA21, and T25) examined in this study were also clearly differentiated by ME‐PFSG within 30 s in a single run without any loss of resolution. The ME‐PFSG technique is a powerful tool for the ultra‐fast detection and simultaneous analysis of GMOs in a variety of foods including maize.