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Temperature gradient focusing in a PDMS/glass hybrid microfluidic chip
Author(s) -
Matsui Takuya,
Franzke Joachim,
Manz Andreas,
Janasek Dirk
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700272
Subject(s) - microfluidics , temperature gradient , microchannel , materials science , ionic strength , capillary action , buffer (optical fiber) , electric field , lab on a chip , electrophoresis , analytical chemistry (journal) , capillary electrophoresis , analyte , chip , concentration gradient , chromatography , nanotechnology , composite material , chemistry , aqueous solution , electrical engineering , physics , engineering , quantum mechanics
This paper reports the application of temperature gradient focusing (TGF) in a PDMS/glass hybrid microfluidic chip. With TGF, by the combination of a temperature gradient along a microchannel, an applied electric field, and a buffer with a temperature‐dependent ionic strength, analytes are focused by balancing their electrophoretic velocities against the bulk velocity of the buffer containing the analytes. In this work, Oregon Green 488 carboxylic acid was concentrated approximately 30 times as high as the initial concentration in 45 s at moderate electric strength of 70 V/cm and a temperature gradient of 55°C across the PDMS/glass hybrid microfluidic chip with a 1 cm long capillary.