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Ionic liquid‐assisted PDMS microchannel modification for efficiently resolving fluorescent dye and protein adsorption
Author(s) -
Xu Yuanhong,
Jiang Hong,
Wang Erkang
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700261
Subject(s) - microchannel , analyte , electrolyte , rhodamine , adsorption , stacking , ionic liquid , surface modification , rhodamine b , chemistry , chromatography , protein adsorption , fluorescence , bodipy , electrophoresis , monolith , materials science , nanotechnology , organic chemistry , electrode , physics , quantum mechanics , photocatalysis , catalysis
Herein, a hybrid system consisting of ionic liquid (IL) and nonionic surfactant has been successfully developed for dynamic modification of PDMS microchips and analyte adsorption such as fluoresent dyes and proteins has been efficiently suppressed. Mutual authentication between microchip electrophoresis and confocal laser scanning microscope was carried out to characterize the multiple novel functions of the IL‐containing system and a possible mechanism was raised. Soluble IL used herein not only played the role as supporting electrolyte, but also provided increased EOF in the PDMS microchannel compared with common electrolytes such as phosphate buffer. Due to the high ionic conductivity of IL, on‐column field‐amplified sample stacking effect was four‐fold higher than that without IL. Furthermore, an excellent synergistic effect existed between IL and nonionic surfactant, which enhanced the ability of resolving analyte adsorption to PDMS surface, and was demonstrated in the sensitive and efficient determination of rhodamine B (with detection limit of 8 nM) and a well separated mixture of proteins.