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Gel‐based proteomics reveals potential novel protein markers of ozone stress in leaves of cultivated bean and maize species of Panama
Author(s) -
Torres Nilka Lineth,
Cho Kyoungwon,
Shibato Junko,
Hirano Misato,
Kubo Akihiro,
Masuo Yoshinori,
Iwahashi Hitoshi,
Jwa NamSoo,
Agrawal Ganesh Kumar,
Rakwal Randeep
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700219
Subject(s) - panama , biology , proteomics , ozone , botany , chemistry , ecology , biochemistry , gene , organic chemistry
We examined responses of cultivated bean ( Phaseolus vulgaris L. cv. IDIAP R‐3) and maize ( Zea mays L. cv. Guarare 8128) plants exposed to ozone (O 3 ) using a leaf injury assessment and proteomics approach. Plants grown for 16 days in greenhouse were transferred to an O 3 chamber and exposed continuously to 0.2 ppm O 3 or filtered pollutant‐free air for up to 72 h. CBB‐stained gels revealed changes in ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCO) protein. By Western analysis changes in marker proteins for O 3 damage in leaves by 1‐DE were checked. In bean leaves, two superoxide dismutase (SOD) protein (19 and 20 kDa) were dramatically decreased, while ascorbate peroxidase (APX, 25 kDa), small heat shock protein (HSP, 33 kDa), and a naringenin‐7‐ O ‐methyltransferase (NOMT, 42 kDa) were increased by O 3 . In maize leaves, expression levels of catalase (increased), SOD (decreased), and APX (increased) were drastically changed by O 3 depending on the leaf stage, whereas crossreacting HSPs (24 and 30 kDa) and NOMT (41 kDa) proteins were strongly increased in O 3 ‐stressed younger leaves. These results indicated a clear modulation of oxidative stress‐, heat shock‐, and secondary metabolism‐related proteins by O 3 . Finally, 2‐DE at 72 h after O 3 exposure revealed changes (induction/suppression) in expression levels of 25 and 12 protein spots in bean and maize leaves, respectively. Out of these, ten and nine nonredundant proteins in bean and maize, respectively, were identified by MS. A novel pathogenesis‐related protein 2 may serve as a potential marker for O 3 stress in bean.