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Enhanced pH‐mediated stacking of anions for CE incorporating a dynamic pH junction
Author(s) -
Arnett Stacy D.,
Lunte Craig E.
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700215
Subject(s) - stacking , ionic strength , analyte , electrokinetic phenomena , chemistry , electrophoresis , ionic bonding , analytical chemistry (journal) , degradation (telecommunications) , base (topology) , resolution (logic) , chromatography , ion , telecommunications , mathematical analysis , mathematics , organic chemistry , aqueous solution , computer science , artificial intelligence
A technique has been developed to enhance analyte focusing for CE for the analysis of physiological samples. High‐ionic‐strength samples are titrated to low‐ionic‐strength on‐line using pH‐mediated sample stacking in conjunction with a dynamic pH junction. This method concentrates analytes by reducing their electrophoretic mobility during field‐amplification. Parameters responsible for enhanced focusing were investigated, and an enhanced pH‐mediated stacking method was optimized for anionic nucleosides. The process results in ultra‐narrow peak widths, for example, 0.28 s for thymidine with a 10 min analysis time. Peak width and resolution with the enhanced stacking method were also compared to normal base stacking and electrokinetic injection. With this technique, mass‐loading capacity can be increased without degradation in peak shape and resolution is dramatically improved.