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Determination of tocopherols in vegetable oils by CEC using methacrylate ester‐based monolithic columns
Author(s) -
LermaGarcía María Jesús,
SimóAlfonso Ernesto F.,
RamisRamos Guillermo,
HerreroMartínez José Manuel
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700195
Subject(s) - chemistry , chromatography , hydroxymethyl , methacrylate , monolith , aqueous solution , monolithic hplc column , polymerization , resolution (logic) , high performance liquid chromatography , organic chemistry , polymer , artificial intelligence , computer science , catalysis
The separation and determination of tocopherols (Ts) in vegetable oils by CEC using methacrylate ester‐based monolithic columns has been developed. The effects of pore size of the monolithic columns were studied, and the composition of mobile phase was optimized. The optimal pore size of the monolith was obtained with 12 wt% 1,4‐butanediol in the polymerization mixture. Excellent resolution between tocopherols was achieved within 10 min analysis time with a 99:1 v/v MeOH–aqueous buffer containing 5 mM tris(hydroxymethyl)aminomethane at pH 8.0. The LODs were lower than 2.3 μg/mL, and interday and column‐to‐column reproducibilities at 25 μg/mL were better than 5.6%. Using a 93:7 v/v MeOH–aqueous buffer, both tocopherols and tocotrienols (T 3 s) of grapeseed and palm oils were resolved. Application to the detection of olive oil adulteration with low‐cost edible oils was demonstrated.