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Development of a CE method for the determination of mycophenolic acid in human plasma: A comparison with HPLC
Author(s) -
Carlucci Filippo,
Anzini Maurizio,
Rovini Michele,
Cattaneo Dario,
Merlini Simona,
Tabucchi Antonella
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700190
Subject(s) - mycophenolic acid , chromatography , human plasma , high performance liquid chromatography , chemistry , plasma , physics , transplantation , medicine , surgery , quantum mechanics
Mycophenolate mofetil (MMF) is a widely used drug for the maintenance of immunosuppressive therapy in renal‐transplant recipients. MMF is rapidly metabolized in vivo to mycophenolic acid (MPA), a reversible, noncompetitive inhibitor of inosine monophosphate dehydrogenase, which represents a limiting enzyme in lymphocyte proliferation. MPA shows large interindividual pharmacokinetic variability: its monitoring is therefore of primary importance to achieve adequate immunosuppression with minimized risk of graft rejection or toxicity. We developed a CE method for the determination of total MPA (tMPA) in plasma, based on easy sample preparation; CE evaluation of tMPA was performed in 30 mmol/L sodium‐borate with 10 mmol/L SDS (pH 10.00) at 25°C using a 60 cm (54.5 to window) uncoated capillary with UV detection at 254 nm wavelength. MPA was readily detectable in plasma; the CE method was linear in the range of 0.7–120 μg/mL ( r >0.992). Intra‐ and interassay imprecision was <7% except for the lowest spiked MPA concentration, which had an intra‐assay RSD% of 14.7 compared to 18.3 interassay. Data by CE were compared with results obtained by a validated HPLC method. CE assay of tMPA exhibited a very good correlation ( r 2 >0.988) with respect to HPLC; Bland–Altman difference versus average showed a mean of −0.18 μg/mL ± 1.14 SD. CE determination of tMPA is a robust, sensitive and reproducible method with the advantage over HPLC of being fast, simple and unexpensive, also enabling quick assessment of MPA for pharmacokinetic studies.