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Determination of glyoxal and methylglyoxal in the serum of diabetic patients by MEKC using stilbenediamine as derivatizing reagent
Author(s) -
Mirza Muhammad A.,
Kandhro Abdul J.,
Memon Saima Q.,
Khuhawar Muhammad Y.,
Arain Rafee
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700129
Subject(s) - methylglyoxal , glyoxal , chemistry , chromatography , reagent , micellar electrokinetic chromatography , standard addition , detection limit , matrix (chemical analysis) , derivatization , high performance liquid chromatography , biochemistry , organic chemistry , enzyme
An analytical method has been developed for the separation of glyoxal (Go), methylglyoxal (MGo), and dimethylglyoxal (DMGo) by MEKC using stilbenediamine (SD) as derivatizing reagent, separation time 6.5 min, SDS as micellar medium at pH 8, and sodium tetraborate (0.1 M) as buffer. Uncoated fused‐silica capillary, effective length 50 cm×75 μm id; applied voltage 20 kV and photodiode array detection, were used. Calibration was linear within 0.02–150 μg/mL with detection limits 3.5–5.8 ng/mL. Go and MGo, observed for diabetic and healthy volunteers, were within 0.098–0.193 μg/mL Go and 0.106–0.245 μg/mL MGo with RSD 1.6–3.5 and 1.7–3.4%, respectively, in diabetics against 0.016–0.046 μg/mL Go and 0.021–0.066 μg/mL MGo with RSDs 1.5–3.5 and 1.4–3.6%, respectively, in healthy volunteers. Go and MGo in diabetics were also measured by standard addition and DMGo as an internal standard. Additives do not contribute significantly to Go and MGo matrix.