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Comparison of resolution in microemulsion EKC and MEKC employing suppressed electroosmosis: Application to bisphenol‐A‐diglycidyl ether and its derivatives
Author(s) -
Poouthree Kieatsuda,
Soonthorntantikul Wasura,
Leepipatpiboon Natchanun,
Petsom Amorn,
Nhujak Thumnoon
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700113
Subject(s) - microemulsion , resolution (logic) , ether , analyte , chemistry , diglycidyl ether , chromatography , bisphenol a , bar (unit) , detection limit , analytical chemistry (journal) , epoxy , organic chemistry , pulmonary surfactant , biochemistry , artificial intelligence , computer science , physics , meteorology
The resolution ( R s ) of hydrophobic analytes in microemulsion EKC (MEEKC) and MEKC with suppressed electroosmosis was investigated using bisphenol‐A‐diglycidyl ether and its derivatives (BADGEs) as test analytes. Separation scales were compared using our equation for the resolution, $\displaystyle {\tf="PS_SLFIT"R}_{{s}} {{ = }}(\sqrt {\bar {\tf="PS_SLFIT"N}} /4)(\alpha - 1)/(1 + {\tf="PS_SLFIT"k}_2 )$ , where k is the retention factor, α the selectivity (α = k 2 / k 1 for k 2 ≥ k 1 >0), and $\displaystyle \bar {\tf="PS_SLFIT"N}$ the average efficiency. At given concentrations of SDS and organic cosolvent in the buffer, in comparison with MEKC, MEEKC was found to provide better resolution of BADGEs, mainly due to the significantly smaller k in MEEKC, but not the greater α in MEEKC, while a comparable range of $\displaystyle \bar {\tf="PS_SLFIT"N}$ . Significantly improved resolution of BADGEs was obtained with increase in the concentration of organic cosolvent in the MEEKC and MEKC buffers, while small change in R s with the SDS concentration in a range of 100–180 mM. In addition, a decrease in temperature or voltage resulted in slightly better R s .