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Channel wall coating on a poly‐(methyl methacrylate) CE microchip by thermal immobilization of a cellulose derivative for size‐based protein separation
Author(s) -
Okada Hiroki,
Kaji Noritada,
Tokeshi Manabu,
Baba Yoshinobu
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700105
Subject(s) - cellulose , coating , materials science , derivative (finance) , substrate (aquarium) , methyl cellulose , methyl methacrylate , methacrylate , chemical engineering , chromatography , polymer chemistry , chemistry , polymer , composite material , copolymer , oceanography , financial economics , engineering , economics , geology
We demonstrate channel wall coating using a cellulose derivative on a poly‐(methyl methacrylate) (PMMA) CE microchip to eliminate EOF disturbing protein separation. The channel walls were modified by preconditioning with a solution containing the cellulose derivative and then thermally evaporating the solution to produce hydrophilic channel walls which prevent adsorption of analytes via a hydrophobic interaction. When the PMMA substrate was coated with the cellulose derivative hydroxypropylmethylcellulose (HPMC) 90SH, the water contact angle on the coated substrate was decreased (up to 15°) and EOF was significantly suppressed (up to 4.0×10 −6 cm 2 ⋅V –1 ⋅s –1 ). Three proteins (20.5, 68.0, and 114.6 kDa) were successfully separated on the 0.15% HPMC 90SH‐coated channel walls with good reproducibility of migration time (RSD <1.75%) and high efficiency (theoretical plate number per meter: 2.62×10 5 ).