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Dynamic modification of poly(methyl methacrylate) chips using poly(vinyl alcohol) for glycosaminoglycan disaccharide isomer separation
Author(s) -
Zhang Yong,
Ping Guichen,
Kaji Noritada,
Tokeshi Manabu,
Baba Yoshinobu
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700088
Subject(s) - vinyl alcohol , disaccharide , methyl methacrylate , polymer chemistry , adsorption , chemistry , methacrylate , poly(methyl methacrylate) , wetting , surface modification , methacrylic acid , contact angle , polymer , chemical engineering , materials science , organic chemistry , polymerization , composite material , engineering
We describe a microchip electrophoresis (MCE) method for the assay of unsaturated disaccharides of chondroitin sulfates, dermatan sulfates, and hyaluronic acid (HA). Poly(vinyl alcohol) (PVA) could be irreversibly adsorbed onto poly(methyl methacrylate) (PMMA) substrates and this approach was applicable for dynamic coating. The characteristics of the PMMA surface with PVA coating were evaluated in terms of the wettability, EOF, and adsorption of 2‐aminoacridone (AMAC)‐labeled disaccharide. The water contact angle decreased from ˜73° on a pristine PMMA surface to ˜37.5° on a PVA‐coated surface, indicating that the PVA coating increased hydrophilicity. EOF was reduced approximately twofold and was relatively stable. Scanning electron microscopy and fluorescence microscopy images showed that adsorption of AMAC‐labeled disaccharides was dramatically suppressed. Using the PVA coating, baseline separation of two pairs of glycosaminoglycan (GAG) disaccharide isomers, ΔDi‐diS B /ΔDi‐diS D and ΔDi‐0S/ΔDi‐HA, was achieved in Tris‐borate buffer within 130 s by MCE.