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Enantioseparation of chiral tropa alkaloids by means of cyclodextrin‐modified microemulsion electrokinetic chromatography
Author(s) -
Bitar Yaser,
Holzgrabe Ulrike
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700008
Subject(s) - microemulsion , chemistry , chromatography , electrokinetic phenomena , micellar electrokinetic chromatography , octane , cyclodextrin , methanol , capillary electrophoresis , organic chemistry , pulmonary surfactant , biochemistry
CD‐modified microemulsion EKC as a CE technique has been applied to the chiral separation of atropine, scopolamine, ipratropium and homatropine. Enantioseparations of these tropa alkaloids were optimized by using a standard oil‐in‐water ( O / W ) microemulsion and varying the nature and concentration of CD additives as well as of the organic modifier (methanol, 2‐propanol or ACN) whilst keeping the applied voltage of 15 kV and capillary temperature of 30°C constant. The standard (O/W) microemulsion BGE solution consisted of 0.8% w/w octane, 6.6% w/w 1‐butanol, 2.0% w/w SDS and 90.6% w/w 10 mM sodium tetraborate buffer (pH 9.2). Enantioseparations with high resolution and short migration times of all tropa alkaloids were achieved by using heptakis(2,3‐di‐ O ‐methyl‐6‐ O ‐sulfo)‐β‐CD and sulfated β‐CD in the microemulsion BGE and were superior to corresponding CD‐modified CE methods.