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In‐channel atom‐transfer radical polymerization of thermoset polyester microfluidic devices for bioanalytical applications
Author(s) -
Pan Tao,
Fiorini Gina S.,
Chiu Daniel T.,
Woolley Adam T.
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600817
Subject(s) - atom transfer radical polymerization , ethylene glycol , capillary electrophoresis , microchannel , peg ratio , thermosetting polymer , polyester , protein adsorption , polymer , chemistry , surface modification , materials science , polymerization , chemical engineering , contact angle , polymer chemistry , nanotechnology , chromatography , organic chemistry , finance , engineering , economics , composite material
Abstract A new technique for polymer microchannel surface modification, called in‐channel atom‐transfer radical polymerization, has been developed and applied in the surface derivatization of thermoset polyester (TPE) microdevices with poly(ethylene glycol) (PEG). X‐ray photoelectron spectroscopy, electroosmotic flow (EOF), and contact angle measurements indicate that PEG has been grafted on the TPE surface. Moreover, PEG‐modified microchannels have much lower and more pH‐stable EOF, more hydrophilic surfaces and reduced nonspecific protein adsorption. Capillary electrophoresis separation of amino acid and peptide mixtures in these PEG‐modified TPE microchips had good reproducibility. Phosducin‐like protein and phosphorylated phosducin‐like protein were also separated to measure the phosphorylation efficiency. Our results indicate that PEG‐grafted TPE microchips have broad potential application in biomolecular analysis.