Premium
Determination of neutral carbohydrates by CZE with direct UV detection
Author(s) -
Rovio Stella,
YliKauhaluoma Jari,
Sirén Heli
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600783
Subject(s) - chemistry , chromatography , rhamnose , xylose , monosaccharide , arabinose , xylitol , calibration curve , sodium hydroxide , detection limit , orange juice , analytical chemistry (journal) , mannitol , mannose , organic chemistry , food science , fermentation
Abstract A new CZE method relying on in‐capillary reaction and direct UV detection at the wavelength 270 nm is presented for the simultaneous separation of the neutral carbohydrates xylitol, D ‐(−)‐mannitol, sucrose, D ‐(+)‐fucose, D ‐(+)‐cellobiose, D ‐(+)‐galactose, D ‐(+)‐glucose, L ‐rhamnose, D ‐(+)‐mannose, D ‐(−)‐arabinose, D ‐(+)‐xylose, and D ‐(−)‐ribose. The alkaline electrolyte solution was prepared of 130 mM sodium hydroxide and 36 mM disodium hydrogen phosphate dihydrate. Separation of the sample mixture was achieved within 35 min. Calibration plots were linear in the range of 0.05–3 mM. Reproducibility of migration times was between 0.3 and 1.1%, and the detection limits for the analytes were 0.02 and 0.05 mM. The optimized method was applied for the determination of neutral monosaccharides in lemon, pineapple, and orange juices and a cognac sample. The methodology is fast since no other sample preparation except dilution is required.