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Low EOF rate measurement based on constant effective mobility in microchip CE
Author(s) -
Wang Wei,
Zhao Liang,
Zhou Fang,
Zhang JianRong,
Zhu JunJie,
Chen HongYuan
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600781
Subject(s) - analyte , analytical chemistry (journal) , chemistry , constant (computer programming) , materials science , chromatography , computer science , programming language
A new method for quickly determining low EOF rates ( μ EOF ) in microchip CE is described. The measurement is based on the notion that the effective mobility ( μ eff ) of an analyte is a constant in a certain BGE. The μ eff of an analyte is determined in a reference fast‐electroosmosis microchip, and the apparent mobility ( μ app ) of the analyte can be determined in the microchip with unknown low electroosmosis, and then μ EOF in the low‐electroosmosis microchip can be calculated according to the equation μ EOF = μ app − μ eff . By an indirect method or other conventional methods, μ eff can be easily measured in the reference microchip. The proposed method is particularly useful for low‐electroosmosis measurements in wall‐modified microchannels.