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Cationic gemini surfactant as dynamic coating in CE for the control of EOF and wall adsorption
Author(s) -
Liu Qian,
Li Yanqing,
Tang Fei,
Ding Li,
Yao Shouzhuo
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600754
Subject(s) - pulmonary surfactant , cationic polymerization , adsorption , repeatability , chemistry , coating , chromatography , chemical engineering , buffer (optical fiber) , polymer chemistry , organic chemistry , biochemistry , computer science , engineering , telecommunications
Abstract The cationic gemini surfactant ethylene bis(1‐dodecyldimethylammonium) dibromide was used as a dynamic coating to control EOF and prevent wall adsorption of basic proteins in CE for the first time. This gemini surfactant shows a more powerful capability in EOF reversal than traditional single‐chained surfactant. The gemini surfactant reverses the EOF at a concentration level even less than 0.01 mM, and the EOF magnitude is affected by surfactant concentration, pH, ionic strength, and ions added in buffer. Highly efficient and rapid protein separation ( N >300 000) was obtained with buffer containing 2 mM gemini surfactant under pH ranging from 3 to 6. The effects of surfactant and buffer concentration on protein separation were investigated in detail. Under the optimal conditions, good repeatability (RSD of migration time <0.6% for run‐to‐run and <2.5% for day‐to‐day assays) and recovery (>90%) of tested proteins were obtained. This new dynamic coating is also suitable for biosample analysis.