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Enantioselective separation of the novel antidepressant mirtazapine and its main metabolites by CEC
Author(s) -
Aturki Zeineb,
Scotti Valentina,
D'Orazio Giovanni,
Rocco Anna,
Raggi Maria Augusta,
Fanali Salvatore
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600731
Subject(s) - chromatography , enantiomer , chemistry , ammonium acetate , capillary electrophoresis , resolution (logic) , analytical chemistry (journal) , high performance liquid chromatography , organic chemistry , artificial intelligence , computer science
In this work, the simultaneous enantioseparation of the second‐generation antidepressant drug mirtazapine and its main metabolites 8‐hydroxymirtazapine and N ‐desmethylmirtazapine by chiral CEC is reported. The separation of all enantiomers under study was achieved employing a capillary column packed with a vancomycin‐modified diol stationary phase. With the aim to optimize the separation of the three pairs of enantiomers in the same run, different experimental parameters were studied including the mobile phase composition (buffer concentration and pH, organic modifier type and ratio, and water content), stationary phase composition, and capillary temperature. A capillary column packed with vancomycin mixed with silica particles in the ratio (3:1) and a mobile phase composed of 100 mM ammonium acetate buffer (pH 6)/H 2 O/MeOH/ACN (5:15:30:50, by vol.) allowed the complete enantioresolution of each pair of enantiomers but not the simultaneous separation of all the studied compounds. For this purpose, a packing bed composed of vancomycin‐CSP only was tested and the baseline resolution of the three couples of enantiomers was achieved in a single run in less than 30 min, setting the applied voltage and temperature at 25 kV and 20°C, respectively. In order to show the potential applicability of the developed CEC method to biomedical analysis, a study concerning precision, sensitivity, and linearity was performed. The method was then applied to the separation of the enantiomers in a human urine sample spiked with the studied compounds after suitable SPE procedure with strong cation‐exchange (SCX) cartridges.