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Analysis of fullerene‐based nanomaterial in serum matrix by CE
Author(s) -
Chan King C.,
Patri Anil K.,
Veenstra Timothy D.,
McNeil Scott E.,
Issaq Haleem J.
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600724
Subject(s) - nanoparticle , detection limit , matrix (chemical analysis) , chemistry , chromatography , resolution (logic) , capillary electrophoresis , biodistribution , micelle , fullerene , nanomaterials , analytical chemistry (journal) , nanotechnology , materials science , biochemistry , in vitro , organic chemistry , artificial intelligence , aqueous solution , computer science
With the increasing interest in using nanoparticles as vehicles for drug delivery and image contrast agents, there is a need to develop assays for their detection and quantitation in complex matrices to facilitate monitoring their biodistribution. In this study, we developed a CE approach for the analysis of two nanoparticles: carboxyfullerene (C3) and dendrofullerene (DF1) in both standard solutions and a serum matrix. These highly soluble, charged C 60 derivatives were characterized by CZE using either a bare or dynamically coated fused‐silica capillaries. The resolution of both nanoparticles was slightly lower with the coated capillary; however, their migration times were faster. While separation of the DF1 nanoparticles using MEKC resulted in a greater number of observable peaks, the peak profile of C3 was basically unchanged regardless of whether SDS micelles were added to the running buffers or not. The MEKC and/or CZE assays were then used to quantitate the C3 and DF1 nanoparticles in spiked human serum samples. The quantitation of the nanoparticles was linear from 0–500 μg/mL with detection limits ranging from 0.5 to 6 μg/mL.