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Determination of 1‐methylhistidine and 3‐methylhistidine by capillary and chip electrophoresis with contactless conductivity detection
Author(s) -
Tůma Petr,
Samcová Eva,
Opekar František,
Jurka Vlastimil,
Štulík Karel
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600697
Subject(s) - capillary electrophoresis , chemistry , chromatography , analytical chemistry (journal) , detection limit , acetic acid , analyte , conductivity , biochemistry
CE with capacitively coupled contactless detection (C 4 D) was used to determine 3‐methylhistidine (3‐MH) and 1‐methylhistidine (1‐MH). The C 4 D response to 3‐MH was studied in a BGE consisting of 500 mM acetic acid and ammonia at varying concentration and the results were compared with the theory. Complete separation of a model mixture of 3‐MH, 1‐MH, and histidine (His) was attained in two optimized BGEs, one containing 500 mM HAc, 20 mM NH 4 OH, and 0.1 % m/v hydroxyethylcellulose (HEC), pH 3.4 (I) and the other consisting of 100 mM morpholinoethanesulfonic acid (MES), 25 mM LiOH, and 0.1 % m/v HEC, pH 5.5 (II). These optimized BGEs were tested in CE/C 4 D analyses of urine. Promising results were obtained for separation and determination of 3‐MH, 1‐MH, and His on a silicon microchip, using aluminum strips as the C 4 D electrodes; the three analytes were baseline‐separated within less than 30 s with a separation channel effective length of 38 mm. The LOD were satisfactory and amounted to 26.4 μM for 3‐MH and 18.3 μM for 1‐MH.