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Investigation of β‐CD‐derivatized erythromycin as chiral selector in CE
Author(s) -
Dai Rongji,
Nie Xiaoying,
Li Hong,
Saeed M. Khalid,
Deng Yulin,
Yao Guowei
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600651
Subject(s) - chemistry , resolution (logic) , erythromycin , chiral derivatizing agent , combinatorial chemistry , chromatography , enantiomer , stereochemistry , chiral column chromatography , antibiotics , biochemistry , artificial intelligence , computer science
A novel water‐soluble β‐CD‐derivatized erythromycin (EM) was synthesized and used as an effective chiral selector for the resolution of chiral compounds in CZE. The purpose of substitution at the primary hydroxyl site of β‐CD with 1‐oxygen‐2,3‐epoxypropane is to produce a compound having functions of both β‐CD and EM. β‐CD‐derivatized EM exhibited excellent enantioselectivities compared with single β‐CD and EM for chiral separation in CE. We also investigated the influence of pH and concentration of BGE, concentration of chiral selector, applied potential, and organic modifier on chiral compounds' separation.