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Expression of high‐abundance proteins in sera of patients with endometrial and cervical cancers: Analysis using 2‐DE with silver staining and lectin detection methods
Author(s) -
AbdulRahman Puteri S.,
Lim BoonKiong,
Hashim Onn H.
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600629
Subject(s) - clusterin , lectin , biology , immunohistochemistry , glycoprotein , downregulation and upregulation , microbiology and biotechnology , silver stain , pathology , medicine , immunology , gene , biochemistry , apoptosis
The expression of high‐abundance serum proteins in newly diagnosed patients with endometrial adenocarcinoma (EACa), squamous cell cervical carcinoma (SCCa) and cervical adenocarcinoma (ACCa), relative to control female subjects, was analyzed by subjecting serum samples to 2‐DE followed by image analysis of the silver‐stained protein profiles. The three cohorts of cancer patients demonstrated different altered expression of serum high‐abundance proteins compared to negative control women. The expression of α 1 ‐antitrypsin, α 1 ‐B glycoprotein, cleaved high‐molecular‐weight kininogen (light chain) and antithrombin III were consistently altered in all the patients. However, clusterin was upregulated only in the patients with EACa, while those with SCCa and ACCa were typically characterized by the upregulated expression of zinc α‐2‐glycoprotein. The aberrant expression of selective serum proteins in the various cohorts of cancer patients was validated by competitive ELISA as well as by lectin detection. Analysis by using the champedak galactose binding lectin further highlighted an unidentified protein that may be differently glycosylated in the sera of the EACa patients that were studied.