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A hybrid microdevice with a thin PDMS membrane on the detection window for UV absorbance detection
Author(s) -
Ma Bo,
Zhou Xiaomian,
Wang Gang,
Dai Zhongpeng,
Qin Jianhua,
Lin Bingcheng
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600619
Subject(s) - absorbance , materials science , detection limit , polydimethylsiloxane , transmittance , microfluidics , membrane , optoelectronics , wavelength , optics , analytical chemistry (journal) , chromatography , nanotechnology , chemistry , biochemistry , physics
We exploited a PDMS‐quartz hybrid microchip with a thin PDMS membrane on the concave detection window for UV absorbance detection. The thickness of the PDMS membrane is about 100 μm, with high UV transmittance. As compared to a PDMS‐quartz hybrid chip with a common detection window, the proposed one exhibited over an one order of magnitude sensitivity enhancement, and an about two orders of magnitude S/N increase for gastrodin ( p ‐hydroxymethylphenyl‐β‐ D ‐glucopyranoside). In addition, the limit of the detection wavelength has been extended from 240 to 210 nm, which is otherwise impossible for a traditional PDMS‐quartz hybrid microchip. This kind of microchip has the potential for a large range of applications in an integrated microfluidic system with UV detection.