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Determination of sertraline and N ‐desmethylsertraline in human plasma by CE with LIF detection
Author(s) -
Musenga Alessandro,
Kenndler Ernst,
Mercolini Laura,
Amore Mario,
Fanali Salvatore,
Raggi Maria Augusta
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600591
Subject(s) - chemistry , chromatography , sertraline , analyte , derivatization , acetone , extraction (chemistry) , human plasma , high performance liquid chromatography , antidepressant , hippocampus , biology , organic chemistry , neuroscience
A method has been developed for the analysis of the antidepressant drug sertraline together with its main metabolite N ‐desmethylsertraline (DMS) in human plasma. It is based on CE with LIF detection ( λ = 488 nm). A SPE procedure is employed for biological sample pretreatment, followed by a derivatization step with FITC; reboxetine was the internal standard. The effect of CD, acetone and N ‐methyl‐ D ‐glucamine (GLC) as constituents of the BGE for analyte separation was investigated. The final BGE consisted of 20 mM carbonate buffer, pH 9.0, with 2.5 mM heptakis(2,6‐di‐ O ‐methyl)‐β‐CD, 50 mM GLC and 20% v/v acetone. With 30 kV applied voltage, the electrophoretic run is completed in 7.5 min. Linearity was observed in the plasma concentration range from 3.0 to 500 ng/mL for sertraline and 4.0 to 500 ng/mL for DMS. Extraction yield was >97.1%, precision – expressed as RSD% – was <3.7, accuracy (recovery) was >95.6%. Due to its sensitivity and selectivity, the method was suited for the analysis of plasma samples from patients undergoing therapy with sertraline.