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CE – a multifunctional application for clinical diagnosis
Author(s) -
Sniehotta Maike,
Schiffer Eric,
Zürbig Petra,
Novak Jan,
Mischak Harald
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600581
Subject(s) - amplicon , analyte , nucleic acid , mass spectrometry , nanotechnology , chemistry , chromatography , clinical diagnosis , computational biology , materials science , biology , medicine , polymerase chain reaction , biochemistry , clinical psychology , gene
Abstract CE has been used widely as an analytical tool with high separation power taking advantage of size, charge‐to‐size ratio, or isoelectric point of various analytes. In combination with detection methods, such as UV absorption, electrochemical detection, fluorescence, or mass spectrometry (MS), it allows the separation and detection of inorganic and organic ions, as well as complex compounds, such as polypeptides, nucleic acids, including PCR amplicons from viruses or bacteria. Recent interest in identification of biomarkers of diseases using body fluids leads to development of CE‐MS techniques. These applications allowed identification of new potential biomarkers for clinical diagnosis and monitoring of therapeutic interventions. In this report, we present a technical overview of various CE techniques and discuss their applications in clinical medicine.