Premium
Electrochromatography in poly(dimethyl)siloxane microchips using organic monolithic stationary phases
Author(s) -
Faure Karine,
Blas Maximilien,
Yassine Omar,
Delaunay Nathalie,
Crétier Gérard,
Albert Monique,
Rocca JeanLouis
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600566
Subject(s) - photopolymer , monolith , materials science , polymerization , monolithic hplc column , siloxane , electrochromatography , acrylate , monomer , methacrylate , photoinitiator , chemical engineering , polydimethylsiloxane , microfluidics , polymer , capillary electrochromatography , nanotechnology , composite material , chemistry , organic chemistry , catalysis , capillary action , engineering
This paper shows the in situ synthesis of an hexyl acrylate monolith in PDMS microfluidic devices and its subsequent use as stationary phase for electrochromatography on chip. To overcome the ability of PDMS material to absorb organic monomers, surface modification of the enclosed channels was realized by UV‐mediated graft polymerization. This grafting procedure is based on the preliminary adsorption of a photoinitiator onto the PDMS surface and polymerization of charged monomers. Next, hexyl acrylate monoliths were cast in situ using photopolymerization process. The chromatographic behavior of the monolithic column was confirmed by the successful separation of derivatized catecholamines in the PDMS device using a 30 mm effective separation length (100 μm×100 μm section). Efficiencies reached up to 200 000 plates per meter.