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Determination of xanthohumol in hops ( Humulus lupulus L.) by nonaqueous CE
Author(s) -
Kac Javor,
Zakrajšek Jure,
Mlinarič Aleš,
Kreft Samo,
Filipič Metka
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600494
Subject(s) - xanthohumol , humulus lupulus , hop (telecommunications) , chemistry , boric acid , chromatography , methanol , capillary electrophoresis , analytical chemistry (journal) , nuclear chemistry , organic chemistry , food science , computer network , pepper , computer science
Abstract Xanthohumol (XN) is a prenylated chalcone with antimutagenic and anticancer activity from hops. A nonaqueous reverse polarity capillary electrophoretic method for the determination of XN in hop extract was developed and validated. The optimal parameters were a 64.5 cm long fused‐silica capillary with 50 μm id at 25°C; 30 kV negative voltage (anode at detector side of the capillary); nonaqueous buffer with 75 mM NaOH and 50 mM boric acid in methanol; hydrodynamical injection with 10 mbar for 40 s; and detection at 440 nm. XN, isoxanthohumol (IX), colupulone, adlupulone, and n ‐lupulone were well resolved on the electropherogram. The LOD for XN was 0.05 mg/L and RSD for peak area was below 3%. The amount of XN in different samples of hop pellets varied from 0.14 to 0.42%.