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Novel quasi‐interpenetrating network/gold nanoparticles composite matrices for DNA sequencing by CE
Author(s) -
Zhou Dan,
Wang Yanmei,
Zhang Wenlong,
Yang Runmiao,
Shi Ronghua
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600488
Subject(s) - interpenetrating polymer network , differential scanning calorimetry , materials science , composite number , polymer , colloidal gold , matrix (chemical analysis) , viscosity , nanoparticle , chemical engineering , polymer chemistry , nanotechnology , composite material , physics , engineering , thermodynamics
Abstract In order to further improve ssDNA sequencing performances using quasi‐interpenetrating network (quasi‐IPN) as a matrix composed of linear polyacrylamide (LPA) with lower viscosity‐average molecular mass (3.3 MDa) and poly( N , N ‐dimethylacrylamide) (PDMA), gold nanoparticles (GNPs) were prepared and added into this quasi‐IPN to form polymer/metal composite sieving matrices. The studies of intrinsic viscosity and differential scanning calorimetry (DSC) on quasi‐IPN and quasi‐IPN/GNPs indicate that there were interactions between GNPs and polymer chains. The sequencing performances on ssDNA using quasi‐IPN and quasi‐IPN/GNPs (with different GNPs concentrations) as sieving matrices were studied and compared by CE at different temperatures. The results show that resolutions of quasi‐IPN/GNPs were higher than those of quasi‐IPN without GNPs and approximated those of quasi‐IPN composed of LPA with higher MW (6.5 MDa) and PDMA without GNPs in the bare fused‐silica capillaries. Furthermore, the sequencing time of quasi‐IPN/GNPs was shorter than that of quasi‐IPN under the same sequencing conditions. The influences of GNPs and sequencing temperature on the sequencing performances of ssDNA were also discussed. The separation reproducibility of quasi‐IPN/GNPs solution was excellent and its shelf life was more than 8 months.