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Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection
Author(s) -
Liu Shaofeng,
Zhang Xue,
Lin Xucong,
Wu Xiaoping,
Fu Fengfu,
Xie Zenghong
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600486
Subject(s) - amperometry , triethylamine , formic acid , chromatography , chemistry , acetone , analyte , detection limit , electrochemistry , electrode , organic chemistry
A new analytical method, pressurized CEC (pCEC) with amperometric detection (AD) using 1.5 μm RP nonporous silica packed columns has been developed for the rapid separation and determination of four Sudan dyes in hot chilli. The influence of several experimental parameters on the retention behavior has been investigated. The electrochemical oxidation of Sudans I–IV separated by pCEC can be reliably monitored with a carbon electrode at +0.95 V ( vs. Ag/AgCl). Fast and efficient separation of the analytes was achieved within 7 min by pCEC under the optimum conditions with an ACN/water (95:5%) mobile phase containing formic acid (pH 4.3), 5% acetone and 0.002% triethylamine using a separation voltage of 12 kV. The detection limits for four Sudan dyes ranged from 8.0 × 10 −7 to 1.2 × 10 −6 mol/L. To evaluate the feasibility and reliability of this method, the proposed pCEC‐AD method was further demonstrated with hot chilli samples spiked with Sudan dyes.