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In‐line liquid‐phase microextraction for selective enrichment and direct electrophoretic analysis of acidic drugs
Author(s) -
Nozal Leonor,
Arce Lourdes,
Simonet Bartolomé M.,
Ríos Ángel,
Valcárcel Miguel
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600468
Subject(s) - chromatography , analyte , extraction (chemistry) , cartridge , capillary electrophoresis , reproducibility , electrophoresis , chemistry , detection limit , materials science , metallurgy
This work describes an efficient in‐line extraction‐preconcentration unit coupled to the electrophoretic capillary based on a liquid‐phase microextraction (LPME) process, which can be directly assembled to the cartridge of the commercial CE equipment. The unit permits analyte extraction, preconcentration and electrophoretic separation to be automatically performed in the commercial CE equipment without the need for additional hardware or software. This new approach was usefully used for the separation and determination of nonsteroidal anti‐inflammatory drugs in human urine permitting at least to analyze 30 consecutive real samples. The LODs were lower than 2 μg/L and the reproducibility, expressed as RSD, was 3.1% for the same unit and only 4.8% between different units.